Single cell sequencing of Tri-PyMT cells [10X Genomics]

Using an EMT lineage-tracing model, we identified tumor cells in the transitioning phases of both EMT and MET. Further analyses revealed that the ribosome biogenesis (RiBi) pathway was specifically elevated in these transitioning cells, regardless of their destined phenotypical fates. Tri-PyMT cells in culture (10th generation) were sorted into RFP+, GFP+ and Double+ cells. Same numbers of subpopulations were remixed and submitted for single cell sequencing with 10X Genomics system.