Supporting data for "Smad3 Inhibition Enhances Neurogenesis and Antidepressant-like Behaviors in Mouse Models"

This dataset contains quantified behavioural data from several experiments, including the characterization of Smad3 knockout animal behavioural phenotype vs wildtypes, the quantification of Smad3 expression in stressed vs non-stressed animals, behavioural outcomes of Smad3 inhibition in the hippocampus and amygdala, as well as Smad3 inhibition with and without TMZ treatment to determine whether the anti-depressant effect of Smad3 inhibition is specific to the hippocampus and whether it is neurogenesis dependent. Quantification of protein expression in Western Blot analysis is included in the dataset and describes the obtained results of non-canonical pathway signalling molecules, flow cytometry data is also included wherein BrdU and GFAP expressing cells were quantified by FACS to determine if Smad3 inhibtion produces any change in the expression of the aforementioned markers.

本数据集包含了多个实验中量化行为数据的集合并详细描述了Smad3敲除动物行为表型与野生型动物的行为特征，以及在应激与非应激动物中Smad3表达的量化。此外，数据集还记录了海马体和杏仁核中Smad3抑制行为结果，以及Smad3抑制与是否接受 TMZ 处理相结合的情况，以确定Smad3抑制的抗抑郁效应是否特异于海马体，以及这一效应是否依赖于神经发生。数据集中还包含了蛋白质表达量的Western Blot分析结果，描述了非典型信号通路分子所获得的结果，并包括流式细胞术数据，其中通过FACS对表达BrdU和GFAP的细胞进行量化，以确定Smad3抑制是否会引起上述标记物表达的改变。