Impact of miR-199a-5p silencing on bleomycin-induced lung fibrosis

To assess the impact of miR-199a-5p silencing on lung fibrogenesis, LNA-miR-199a-5p (5mg/kg) or control formulated for in vivo delivery was instilled intratracheally 4 days and 2 days before intratracheal administration of bleomycin (1 unit/kg) or PBS as well as 4 days after bleomycin or PBS treatment. Experiments were performed in a one color design, corresponding to 4 conditions (LNA-control + PBS n = 3, LNA-miR-199a-5p + PBS n = 3, LNA-control + Bleomycin n = 5, LNA-miR-199a-5p + bleomycin n = 5) for a total of 16 samples.