RNAseq data intron3 freo GTEx

Analysis of RNA-seq data was raw data was obtained from the Genotype-Tissue Expression project (GTEx). A total of 363 samples of frontal cortex, dorsolateral prefrontal cortex, and hippocampus from 180 non-demented human brain donors were analysed. For donors with more than one sample in the same brain region, only the one with the highest levels of MAPT were analysed. FASTQ files were obtained from the SRA files and reads were re-mapped to human genome GRCh38 by means of STAR 2.5.2a. Gene expression was quantified using RSEM 1.3.1, as Transcripts per Million (TPM). The annotation file was obtained from GENCODE v23 and was modified to include TIR12-MAPT gene (coordinates chr17:45894382–46018851), which contains part of intron 12 (coordinates chr17:46018731–46018851) as the 3’ end of the gene.