Deficiency of Irx5 protects mice from obesity and associated metabolic abnormalities

Here we aim to decipher the actions of Irx5 in the regulation of obesity and metabolic abnormalities. We employed a mouse model homozygous for an Irx5-knockout (Irx5KO) allele and conducted droplet based single-cell RNA sequencing (Drop-seq) in the hypothalamic arcuate-median eminence (ARC-ME), which is the main control center for sensing and integrating feeding regulatory signals. Overall design: Drop-seq was perfomed on WT and Irx5KO adult mouse hypothalamic arcuate nucleus-median eminence. Micro-dissected tissues from 5-7 mice per each group were pooled together for single cell dissociation, followed by Drop-seq sequencing.