Metadata record for the manuscript: Anti-tumor effects of an ID antagonist with no observed acquired resistance

Summary

This
metadata record provides details of the data supporting the claims of the
related manuscript: “Anti-tumor effects of an ID
antagonist with no observed acquired resistance”.

The
related study aimed to show that AGX51 treatment of cancer cell lines impairs
cell growth and viability that results from an increase in ROS production upon
ID degradation. (ID proteins are helix-loop-helix (HLH) transcriptional
regulators frequently overexpressed in cancer. ID proteins inhibit basic-HLH
transcription factors often blocking differentiation and sustaining
proliferation. The small-molecule, AGX51, targets ID proteins for degradation
and impairs ocular neovascularisation in mouse models.)

Type of data:
LC-MS/MS and whole proteome analysis; flow cytometry

Subject of
data: Eukaryotic cell lines; Mus musculus

Sample size:
3 replicates per experimental condition for in vitro experiments; 5 mice per
group in each mouse experiment. Sample sizes determined based on an expected large
effect size.

Population
characteristics: Weight measurements and standard blood analyses were carried
out on 8-12 week-old male CD1 mice. Orthotopic mammary fat pad tumors were
generated in 8-12 week-old, female athymic nu/nu mice (Simonsen Laboratories).
Lung metastases were generated by injecting 6-8 week-old, female, Balb/c mice
(Taconic). Spontaneous colon tumors were induced by treating 30, 4-week old
male A/J mice (Jackson Laboratory).

Data
access

The proteomics
data of anti-tumour effects of an ID antagonist with no observed acquired
resistance are openly available in the PRIDE Archive via the following
accession: https://identifiers.org/pride.project:PXD024593.

The raw data
underlying the following figures are openly available as part of this data
record: 1E, 2A, 3B,C,D, 4A,E, 5A,B,F,G 6B, S1A,D,F,G, S2E,I,J S5A,B S6, S7A,
S8C. A comprehensive list of which file is associated with each figure is
included in the Excel spreadsheet ‘Wojnarowicz_et_al_underlying_data_files_list.xlsx’.

All
uncropped Western Blots are in the Supplementary Information section of the
related article.

Corresponding author(s) for this study

Robert
Benezra. Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer
Center, New York, NY. [e-mail].

Ethics

Animal
studies were carried out in accordance with institutional regulations (IACUC
protocol 06-10-025).