Targeted Nanopore Sequencing with Cas9 for studies of methylation, structural variants and mutations

This study sought to explore the use of Cas9 for enrichment of specific loci for targeted nanopore sequencing. We use this data to evaluate methylation state at cancer-driver genes as well as to evaluate structural variations. We also apply use this method to evaluate the ability of nanopore sequencing to call single nucleotide variants from high-coverage sequencing data.