<strong>Immunomodulatory Effects of Pirdot Leaf Ethanol Extract (</strong><em><strong>Saurauia vulcani</strong></em><strong>. Korth ) on Hepatic CD4+ T cell Profile and Serum IL 2 Levels in Wistar Strain Mice Induced </strong><em><strong>Salmonella typhimurium</strong></em>

<strong>Introductions:</strong> Elements of the immune system that contributes to salmonella infection is CD4+ and IL2 T cells. One of the herbs that play a role in immunity is pirdot leaves (Saurauia vulcani Korth) which contains flavonoids that play a role in inflammation. <strong>Aims:</strong> the study to investigated Immunomodulatory Effects of Pirdot Leaf Ethanol Extract (<em>Saurauia vulcani</em>. Korth ) on Hepatic CD4+ T cell Profile and Serum IL 2 Levels in Wistar Strain Mice Induced <em>Salmonella typhimurium.</em> <strong>Methods:</strong> experimental research post-test design only control group. Male wistar rats are divided into 4 groups: I. Normal (KO), II. Negative (Kneg), III. Imboost 22.5 mg/kgBB (KPos), IV. Ethanol extract of pirdot leaves 500mg/kgBB(P). Groups II-IV received s injections. thypimurium on the 4th day. Blood is obtained from the heart and the liver at the conclusion of the killed animal in order to assess the liver's CD4 + T cells, histology, and IL 2 levels. <strong>Results:</strong> The Kruskal-Wallis test and Whitney Man Test were used to examine the data. The results were the highest levels of IL 2 in pirdot (2.23 ±0.18)ng/ml,followed by KPO (1.86 ±0.33)ng/ml,K0 (1.71±0.40)ng/ml, and the lowest at KNeg (1.64±0.28)ng/ml.The average value of histopathological structure score in Kruskal-Wallis test showed significant differences in necrosis hydropic degeneration, inflammation, granuloma, porta triad damage (p&lt;0.05). Meanwhile the comparison of CD4 + T cell IRS score results there were significant differences in all groups p=0.004. <strong>Conclusion:</strong> Pirdot leaf ethanol extract dosage of 500mg/kgBB improved CD4 + T cell IRS scores and serum IL 2 levels compared to other groups.## 引言 参与沙门氏菌感染的免疫系统组分包括CD4+ T细胞及白细胞介素2（Interleukin-2, IL-2）相关T细胞。具有免疫调节活性的草本植物包含皮尔多特叶（*Saurauia vulcani* Korth），其所含黄酮类化合物可参与炎症调控过程。 ## 研究目的 本研究旨在探讨皮尔多特叶乙醇提取物（*Saurauia vulcani* Korth）对鼠伤寒沙门氏菌（*Salmonella typhimurium*）诱导的Wistar大鼠肝脏CD4+ T细胞谱系及血清IL-2水平的免疫调节作用。 ## 研究方法 本研究采用仅设对照组的实验后测设计。将雄性Wistar大鼠随机分为4组：1. 正常对照组（KO）；2. 模型对照组（Kneg）；3. 阳性药物对照组（22.5 mg/kg体重的Imboost，KPos）；4. 皮尔多特叶乙醇提取物组（500 mg/kg体重，P组）。第4天对第2至4组大鼠注射鼠伤寒沙门氏菌。实验结束后处死大鼠，经心脏采血并采集肝脏组织，用于检测肝脏CD4+ T细胞水平、组织病理学特征及血清IL-2水平。 ## 研究结果 本研究采用Kruskal-Wallis检验及曼-惠特尼U检验（Mann-Whitney U test）分析实验数据。结果显示，IL-2水平以P组最高（2.23±0.18）ng/ml，其次为KPos组（1.86±0.33）ng/ml、KO组（1.71±0.40）ng/ml，模型对照组（Kneg）最低（1.64±0.28）ng/ml。Kruskal-Wallis检验结果表明，各组大鼠肝脏组织病理结构评分（涵盖水样变性、炎症反应、肉芽肿形成及门三联管损伤）差异均具有统计学意义（p<0.05）。同时，各组大鼠肝脏CD4+ T细胞免疫反应评分（IRS）比较，差异亦具有统计学意义（p=0.004）。 ## 结论 500 mg/kg体重的皮尔多特叶乙醇提取物可提升大鼠肝脏CD4+ T细胞IRS评分及血清IL-2水平，其效果优于其余各组。