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Genome wide CRISPR screen for CG-806 resistance in OCI-LY3 cells using Y. Kosuke library

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NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE174766
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OCI-LY3 cells were infected with CRISPR/Cas9 library, selected for puromycin resistance for integration events and exposed to CG-806 or vehicle, DMSO, at 1 microMolar concentrations. Cells were collected at time 0 (post puromycin selection) and day 7. DNA was extracted and sgRNA barcodes were amplified. PCR library was deep sequenced using highthroughput Illumina platform Novaseq 6000. Performed comparisons of sgRNA read counts between DMSO and CG-806 treated cells at corresponding time points.
创建时间:
2023-05-04
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