Additional file 1 of Anaerobic derivates of mitochondria and peroxisomes in the free-living amoeba Pelomyxa schiedti revealed by single-cell genomics

Additional file 1: Table S1. Statistics of Pelomyxa schiedti assemblies were compared with those of Mastigamoeba balamuthi. Table S2. Proteins targeted to MRO of Pelomyxa schiedti. Localization of proteins was predicted by several tools, as listed in columns E - J. Mitochondrial predictions are highlighted by white text on blue background. Column K shows final inferred prediction of localization. Abbreviations: cyt, cytosolic; ER, endoplasmic reticulum; extracell, extracellular; mit, mitochondrial; nuc, nuclear; Other, other localization; perox, peroxisomal; PM, plasma membrane; sec, secretory system; SP, signal peptide; -, protein not localized in MRO; +, protein localized in MRO; +?, protein localized in MRO with low confidence. Table S3. Proteins required for peroxisome biogenesis and targeted to peroxisome. Proteins identified in Mastigamoeba balamuthi are highlighted by white text on blue background. Proteins were considered peroxisome-targeted, if they contained PTS1 (SRI or [SAP][KR][LM]) or PTS2 motif (R[LIV](x5)HL), and/or were predicted by PTS1 predictor [83]. Table S4. Primers used in this study.