Effect of NOD1 and TLR4 stimulation on human umbilical vein endothelial cells (HUVEC) and human aortic endothelial cells (HAEC).

HUVEC and HAEC were cultured for 24 hours in 96 well plates with media alone, iE-DAP 10 µg/ml (NOD1 agonist) or LPS 1 µg/ml (TLR4 agonist). CXCL8 release was used as a measure of vessel activation. Normalised results expressed as mean percentage change from control ± SEM for n = 9 (HUVEC) or n = 6 (HAEC). Results were analysed by one-sample t-test (*P<0.05).