An eco-friendly ultra-performance liquidchromatography–mass spectrometry methodfor quantification of rivaroxaban andticagrelor in rat plasma: grapefruitinteractions: supplmentary data

Aim: An eco-friendly ultra-performance liquid chromatography–tandem mass spectrometry methodwas developed to study the pharmacokinetics of rivaroxaban and ticagrelor in rat plasma, utilizingmoxifloxacin as an internal standard. The food–drug interaction between grapefruit juice and these drugswas also investigated. Methods: Liquid–liquid extraction was used. A nonporous stationary phase Agilent R ?Poroshell 120EC C18 column was used with methanol: 0.1% aqueous formic acid (95:5 v/v) as a mobilephase. The detection was performed in multiple reaction monitoring mode using positive electrosprayionization. The method’s validation was conducted in accordance with US FDA and European MedicinesAgency guidelines. Results & conclusion: Grapefruit juice should be ingested with caution in patientstreated with antithrombotic medications as it may increase their plasma concentration, inducing bleeding,and requires close clinical monitoring.