Altered dermal architecture in PKCalpha-/- skin

Global gene expression analysis in PKCalpha-/- mouse skin reveals structural changes in the dermis and defective wound granulation tissue. The skin's mechanical integrity is maintained by an organised and robust dermal extracellular matrix (ECM). Resistance to mechanical disruption hinges primarily on homeostasis of the dermal collagen fibril architecture which is regulated, at least in part, by members of the small leucine-rich proteoglycan (SLRP) family. This array study presents data linking protein kinase C alpha (PKCalpha±) to the regulated expression of multiple ECM components including SLRPs.对蛋白激酶Cα（PKCα）基因敲除（PKCα⁻/⁻）小鼠皮肤开展的全基因表达分析显示，其真皮层存在结构异常，且伤口肉芽组织生成存在缺陷。皮肤的机械完整性由结构规整且稳固的真皮细胞外基质（extracellular matrix, ECM）维持。皮肤抵御机械损伤的能力主要取决于真皮胶原纤维结构的稳态平衡，而该结构至少部分受富含亮氨酸重复序列蛋白聚糖（small leucine-rich proteoglycan, SLRP）家族成员的调控。本基因表达微阵列研究提供了关联蛋白激酶Cα（PKCα）与包括SLRPs在内的多种细胞外基质成分调控表达的实验数据。