SHP-1 dependent dephosphorylation of Histone H2B acts as a molecular switch to facilitate transcription. SHP-1 dependent dephosphorylation of Histone H2B acts as a molecular switch to facilitate transcription

1.Comparison of chromatin occupancy of H2BUb and RNA Polymerase II in SHP-1 WT and KO MCF7 cells. Paired end sequencing (2X100 bp) was performed on the Illumina HiSeq X ten. 2.Identification of the SHP-1 binding site in MCF7 cells using ChIP Seq. Paired end sequencing (2X100 bp) was performed on the Illumina Nextseq 2000 platform.