Transcriptional response of Saccharomyces cerevisiae to lactic acid enantiomers

The model yeast species Saccharomyces cerevisiae is used in many fundamental and applied research applications, including biosensors and production of many compounds. However, given the enormous work invested in the studies of yeast transcription response to various conditions, there are still substances not explored in this regard. In this work, we explore the transcriptional response of S. cerevisiae to a wide range of concentrations of the D-enantiomer of lactic acid and compare it to the response to L-lactic acid. Of these conditions, we only recorded a transcriptional response to the relatively high concentrations of DLA of 5 and 45 mM, as well as to 45 mM LLA. Our data did not reveal any natural yeast promoters that quantitatively sense D-lactic acid but provide the first description of the transcriptome-wide response to DLA, as well as enrich our understanding of the LLA response.模式酵母酿酒酵母（Saccharomyces cerevisiae）已被广泛应用于诸多基础与应用研究场景，涵盖生物传感器开发及多种化合物的合成生产。尽管学界已针对酵母在不同条件下的转录应答开展了大量研究，但仍有诸多物质未被纳入此类探索范畴。本研究中，我们探究了酿酒酵母对不同浓度乳酸D型对映体的转录应答，并将其与对L-乳酸的应答进行对比分析。在本次实验设置的诸多条件中，我们仅观测到酿酒酵母对浓度相对较高的5 mM、45 mM D-乳酸（DLA）以及45 mM L-乳酸（LLA）产生了转录应答。我们的研究数据未发现可定量感知D-乳酸的天然酵母启动子，但首次完成了全转录组水平下D-乳酸应答的系统性描述，并进一步丰富了学界对L-乳酸应答机制的认知。