RNA-seq analysis of human WI-38 fibroblast cell line treated with a chalcone that promotes resistance to oxidative stress

We have identified through high-throughput screen a number of small molecules that promote resistance of human WI-38 cells to hydrogen peroxide. To look into potential mechanism of action for a chalcone hit, we analyzed transcriptional profile by RNA-seq for WI-38 cells treated with this molecule. WI-38 cells were treated with 10uM chalcone (namely, Gr-4D from two different vendors, CB=ChemBridge, MP=MolPort) for 24 hours. Total RNA was isolated using the RNeasy kit (Qiagen) and used to prepare library (TruSeq Stranded mRNA poly A) for RNA-seq analysis (HiSeq 4000).