Major MLL fusion partners regulate promoter-proximal pausing and release of RNA polymerase II

1Sheng Yushou Center of Cell Biology and Immunology, Department of Genetics and Developmental Biology, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. 2Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10065, USA. 3Systems Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA. 4CCTS Bioinformatic Program, The Rockefeller University, New York, NY 10065, USA. 5State Key Laboratory of Genetic Engineering & Ministry of Education Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Fudan University, Shanghai, 200438, China Overall design: Study the roles of major fusion partners of MLL1 in mixed lineage leukemia, including AFF1, AFF4, AF9, and ENL, and H3K79 methyltransferase, DOT1L, in transcriptional regulation by ChIP-seq, RNA-seq, and Pro-seq.