Gene expression analysis in the absence of Creb in Pomc-expressing neurons of the hypothalamus

Brain-derived serotonin favors appetite in mice following its binding to the Htr1a and Htr2b receptors in arcuate neurons of the hypothalamus. In this study, we identified that CREB is the transcriptional effector of brain-derived serotonin control of appetite in arcuate nuclei. In this dataset, we identified the downstream genes of CREB in arcuate neurons of the hypothalamus controling appetite. We isolated hypothalami of wild type and Creb-pomcCre-/- (deleted for Creb selectively in arcuate neurons of the hypothalamus) mice and performed microarray experiments.脑源性血清素（Brain-derived serotonin）与小鼠下丘脑弓状神经元的Htr1a和Htr2b受体结合后，可促进小鼠食欲。本研究确认，CREB是脑源性血清素在弓状核内调控食欲的转录效应分子。本数据集针对调控食欲的下丘脑弓状神经元，鉴定了其中CREB的下游靶基因。我们分别分离了野生型小鼠与Creb-pomcCre-/-小鼠（该品系小鼠的下丘脑弓状神经元中Creb被特异性敲除）的下丘脑组织，并开展了微阵列（microarray）实验。