GNPS - Untargeted Blood Lipidomics Analysis in Critically Ill Pediatric Patients with Ventilator-Associated Pneumonia

Blood samples were collected from 20 patients with suspected ventilator-associated pneumonia (VAP), aged 6 months to 15 years, at days 1, 3, 6, and 12, followed by an untargeted lipidomics analysis using Ultra-High Pressure Liquid Chromatography coupled with High-Resolution Mass Spectrometry (UPLC-HRMS) on a TIMS-TOF/MS platform.
Patients were stratified into high (mCPIS > 6, n = 12) and low (mCPIS < 6, n = 8) VAP suspicion groups based on the modified pediatric clinical pulmonary index score (mCPIS).