Mitochondria-targeted partial inhibiton of complex I increase health and lifespan in mice.

We performed next-generation RNA sequencing (RNA-seq) using brain tissue from 23 months old vehicle and CP2-treated non-transgenic (NTG) and Young (3 months old) WT mice. By comparing transcriptomic data of vehicle and CP2-treated NTG_Old mice, we found processes affected by CP2 such as genes involved in regulation of circadian rhythm, immune system, oxidant detoxifications. In comparison to Young mice, CP2 treatment in NTG_Old mice downregulated the expression of gene sets involved in regulation of oxidative stress and lipid metabolism, which were both upregulated with old age in vehicle-treated NTG_Old mice. mRNA profiles of 23 months old non-transgenic (NTG) and non-treated and CP2 (mitochondrial complex I inhibitor)-treated APP/PS1 mice were generated by deep sequencing, using Illumina HiSeq 4000 with paired end 101-bp read length.