Human HFF cells large rearrangements detection after CRISPR-Cas9 nuclease (Fig. 3b). Human HFF cells large rearrangements detection after CRISPR-Cas9 nuclease (Fig. 3b)

SORSC1 gene editing by CRISPR-Cas9 nuclease in heterozygous HFF for UROS inactivation. Fluorescent positive cell sorting for UROS and large rearrangements confirmation in chromosome 10q. Overall design: One condition experiment, Florescent positive cell bulk HFF vs Fluorescent negative HFF cell bulk after SORSC1 gene editing.