Additional file 1: of Reintroducing testosterone in the db/db mouse partially restores normal glucose metabolism and insulin resistance in a leptin-independent manner

Figure S1. Aromatase inhibitor anastrozole blocks conversion of exogenous testosterone to estradiol (aromatization). The continuous anastrozole infusion rate was adjusted to 0, 20, or 200 μg/day (n = 8–10/group). A significant increase in blood estradiol levels was observed at an exogenous testosterone dose of 100 μg/g body weight/2 days, which were significantly decreased upon anastrozole infusion (20 or 200 μg/day) in castrated db/db mice. Results are expressed as means ± s.e.m. *P < 0.01 versus the other group of castrated db/db mice on the NCD. (ODP 17 kb)