Comparisons of automated Leica <i>HER2</i> FISH with digital analysis and manual Abbott <i>HER2</i> FISH with manual analysis in 20 full-sized slides of tissue specimens used for TMA blocks of invasive breast cancer.

FISH, fluorescence in situ hybridization. a ratio ≥ 2.2. b ratio = 1.8–2.2. c ratio <1.8. d between brackets equivocal ratio after additional count of 20 nuclei <2.0 or ≥2.0. e 3 categories (amplified / equivocal / non-amplified). f 2 categories (amplified ratio ≥ 2.0 / non-amplified ratio <2.0). Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis in 20 full-sized slides of tissue specimens used for TMA blocks of invasive breast cancer.