H3K14ac binding by its Triple Tudor Domain globally directs SETDB1 activity

SETDB1 is a major H3K9 methyltransferase. It contains a unique Triple Tudor Domain (3TD) which specifically bind the dual modification of H3K14ac in the presence of H3K9me1/2/3. In this study, we explored the role of the 3TD H3K14ac interaction in the methylation of H3K9 methylation activity of SETDB1. We performed genome wide DNA methylation profiling using Infinium MethylationEPIC BeadChip (EPIC, Illumina Inc., San Diego, CA, USA) in HCT116 and SETDB1 KO cells and anlyse the role of H3K14ac in SETDB1 dependent DNA methylation. Bisulphite converted genomic DNA isolated from HCT116 wild type and HCT116 SETDB1-knockout cells was hybridized to the Illumina MethylationEPIC Beadchip (biological duplicates).