CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms

Exon Level Expression Profiling of HeLa cells depleted of DNMT1 by 2 independent siRNAs In HeLa cells where depletion of DNMT1 was sufficient to cause reduced levels of DNA methylation inside the body of CD44 and reduced usage of CD44 variant exons, without affecting splicing factors regulating CD44. We analyzed DNMT1-depleted HeLa cells fro using the Affymetrix Human Exon 1.0 ST platform. Array data was processed by Genosplice. Three biological replicates were performed.