The effects of low-doses UVA irradiation on HaCaT keratinocytes

We examined the effects of low-doses UVA irradiation on HaCaT keratinocytes with the aim of identifying UVA-induced effects on cell motility proteins. For this purpose, a comparative proteomic analysis of HaCaT keratinocytes was performed before and after irradiation with two cumulative UVA doses of 5 J/cm2 (UVA1) and 25 J/cm2 (UVA2). A total of 514 common proteins were detected for all three groups of cells. Moreover, more than half of these proteins changed their expression in response to UVA exposure even at low doses. Proteins associated with the biological process "Cell response to stress", (GO:0033554) were most strongly upregulated. 23 proteins associated with the biological process GO:0048870 - "cell motility", which are involved in the adaptive response to stress (e.g. UV radiation), were identified. 20 cell motility proteins were significantly upregulated, of which cytoskeletal actin-binding proteins were the major group. It is hypothesized that changes in the content of cell motility proteins in HaCaT keratinocytes can apparently be used to study physiological and pathological processes, particularly processes during wounding and wound healing.