Immunohistochemical detection of Der p 1, IgE and CD68, and the presence of eosinophil leukocytes in nasal biopsies before and after in vivo challenge with Der p 1 allergen.

H-E staining for Eosinophils; see Methods.*Double staining with Der p 1.** Staining of cells and the mucous in the epithelium, lamina propria and the submucosal glands. An ordinal scale was used for intensity of Der p 1 staining. This allows for rank order (negative, 1+, 2+, 3+, 4+), but does not allow for measuring relative degree of difference between them.***Comparison between non-challenged (pre) and challenged (post) tissue.****Comparison between atopic and nonatopic (non-challenged tissue).EP: nasal mucosal epithelium; LP: lamina propria; GL: subepithelial mucous glands.Number of cells: Mean (range); NA: not available; Statistics: t-test was used for comparison between means.Immunohistochemical detection of Der p 1, IgE and CD68, and the presence of eosinophil leukocytes in nasal biopsies before and after in vivo challenge with Der p 1 allergen.