Transcriptomes of normal liver and spontaneous hepatocellular carcinoma in C3HeB/FeJ male mice. Mus musculus

The goal of this study was to identify and analyze differential gene expression between normal liver and tumor tissue responding or not to potential treatments. A second goal was to compare the mouse results with that from analysis of human differential gene expression for normal liver and hepatocellular carcinoma. Overall design: Mice were allowed to reach 10-months-old then assessed for the presence of tumors. Animals with tumors were subjected to treatment protocols to be described else where. After six 28-day cycles of treatment, mice were euthanized and tissue collected for RNA isolation that was used for next generation sequencing by Beckman Coulter. Results were analyzed using Pigengene package.