Passion fruit cold acclimation transcriptometric analysis Unigene.fasta

Chilling stress (CS) is an
important limiting factor for the growth and development of passion fruit (Passiflora edulis) in winter
in south China. However, little is known about how the passion fruit responds
and adapts to CS. In this study, we performed transcriptome sequencing of
Huangjinguo (HJG, cold-susceptible) and Tainong 1 (TN1, cold-tolerant) under
normal temperature (NT) and CS conditions, and a total of 47,353 unigenes were
obtained in 7 databases. Using differentially expressed unigenes (DEGs)
analysis, 3,248 and 4,340 DEGswere identified at two stages, respectively. The Gene
Ontology (GO) enrichment analysis showed that the DEGs were mainly related to
phosphorylation, membrane protein, and catalytic activity. In Kyoto
Encyclopedia of Genes and Genomes (KEGG) pathway, the unigenes of plant-pathogen
interaction, plant hormone signal transduction and fatty acid metabolism were
enriched.Then, the 12,471 filtered
unigenes were divided into 8 co-expression modules, and two of which were
correlated with CS. In the two modules, 32 hub unigenes were obtained. Furthermore,
the unigenes related to CS were
validated using quantitative real-time
PCR (RT-qPCR). This work showed that the expression levels
of CS-related unigenes were very different in two passion fruit cultivars. The results
provide information for the development of passion fruit with increased chilling
tolerance.