Expression of wild-type twk-16 intron constructs.

Summary of expression in transgenic C. elegans lines. Lines are denoted as wild type (WT) followed by a number with approximate size (bp) of the twk-16 experimental sequence. The total number of animals scored is in parentheses with YFP expressing animals shown as a percentage of the total under the corresponding regions of the nervous system. All constructs (except plasmid WT2000) were made by PCR fusion with the expression vector shown in Figure 2B. The constructs used to generate the transgenic lines were: WT2000 with 500 bp 5′ of exon 1, exon 1 and the 1.4-kb first intron. The experimental sequences used were the following constructs (Figure 5A): WT700 with twk-16.cs1 and twk-16.cs2 regions and flanking sequence; WT350 with twk-16.cs2 region and flanking sequence; WT500 with twk-16 cs.1 region and flanking sequences; WT300 with twk-16.cs1 region and flanking sequence; WT195 with twk-16.cs1 region and flanking sequence; WT113 with twk-16.cs1 region and flanking sequence; WT85 with twk-16.cs1 and short flanking sequence; WT53 with 53 bp of twk-16.cs1; and WT53R is the reverse complement of WT53. The sub-fragments of WT53 are the 5′ 29b bp of WT53 (WT29) and the 3′ 24 bp of WT53 (WT24) (Figure 5B). The only individual neuron scored was DVA. (F signifies faint YFP expression).