Contamination by somatic testis sheath has detectable effects on changes in gene expression between stages of spermatogenesis.

Gene expression differences are log2 fold-change between the various dissections. qRT-PCR values were normalized by three control genes (see Materials and Methods).aPremeiotic dissections with testis sheath included − meiotic dissections.bPremeiotic dissections with testis sheath included − premeiotic dissections with testis sheath removed.cSheath effect is calculated as the ratio of (Ps−Pn)/(Ps−M) from qRT-PCR.dp-value calculated from paired t tests between Ps and Pn.