Transcriptomic analysis for understanding differential gene expression upon FKBP5 knockdown

To investigate differentially expressed genes in HEK293T cells upon FKBP5 knockdown (n =2, reported in this study) and control scramble (n=2) (reported earlier Yadav et al., Cell Reports 2019, NCBI Genbank SRA accession no. PRJNA512165) using total RNA extracted from HEK293T cells and analysed by RNA-seq. We created stable FKBP5 knockdown cells using specific shRNAs. Total RNA were extracted from these cells and used for downstream RNA-Seq analyses.