scRNA-seq of E9.5 para-aortic splanchnopleura from NCX1-null embryos and wild type controls

In order to study the effect of Ncx1 deletion on the HSC-supportive microenviromrent in the AGM niche, caudal parts from E9.5 (20-23 sp) Ncx1-/- and Ncx1+/+ littermate controls (4 embryos/group) were dissected and processed into single cell suspensions and dead/damaged cells were removed by magnetic separation. Resulting cell suspension from Ncx1-/- and Ncx1+/+ embryos loaded onto independent channels of a Chromium chip before single cell partitioning and barcoding on Chromium Controller (10x Genomics).