Fzd6 knockout skin E15.5

The hair follicle misorientation phenotype in Fzd6-/- mice appears to act through the PCP signaling system, but the downstream effectors of Fzd6 remain mysterious. We used microarrays to search for potential downstream effectors of the Fzd6 signaling pathway in regulating hair follicle orientation. We profiled the transcriptomes of back skins from E15.5 Fzd6+/- and Fzd6-/- embryos by hybridization of three independent samples to Affymetrix MOE430 2.0 gene chips.Fzd6纯合敲除（Fzd6-/-）小鼠的毛囊定向异常表型似乎通过平面细胞极性（Planar Cell Polarity, PCP）信号通路发挥作用，但Fzd6的下游效应因子仍不明晰。为此，我们采用基因芯片技术，针对Fzd6信号通路调控毛囊定向过程中的潜在下游效应因子开展筛选。具体而言，我们将三份独立样本与Affymetrix MOE430 2.0基因芯片进行杂交，以此对胚胎发育第15.5天的Fzd6杂合子（Fzd6+/-）与Fzd6纯合敲除（Fzd6-/-）小鼠胚胎的背部皮肤转录组表达谱进行分析。