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METHOD OF PRODUCING RECOMBINANT SUCROSE SYNTHASE, USE THEREOF IN THE PRODUCTION OF SUCROSE-DETERMINATION KITS, METHOD OF PRODUCING ADPGLUCOSE AND METHOD OF OBTAINING TRANSGENIC PLANTS HAVING LEAVES AND RESERVE ORGANS WHICH ACCUMULATE A HIGH CONCENTRATION OF ADPGLUCOSE AND STARCH

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国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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It is provided a method for the efficient production of large quantities of soluble recombinant sucrose synthase (SS) in the active form thereof, with the expression of the gene that codes for SS in a strain of Escherichia coli. The expression vector used enables the recombinant SS thus produced to have a histidine tail which facilitates the rapid purification thereof. In addition, it is provided sequences of mutated versions of the SS gene, which code for SS isoforms that are suitable for the production of ADPG. It is also provided a method of obtaining transgenic plants which overexpress the SS gene, either constituently or in the reserve organs or leaves thereof, and which have a high concentration (both in the leaves and in the reserve tissues) of sucrose, ADPG, G6P and starch owing to the high ADPGsynthesisingactivity of SS.
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国家林业和草原科学数据中心
创建时间:
2023-02-12
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