Dataset to "A hydroquinone-specific screening system for directed P450 evolution"

In direct evolution, an effective screening strategy is crucial for the identification of improved enzymes with desired characteristics in large mutant libraries. In the manuscript “A hydroquinone specific screening system for directed enzyme evolution” (A. M. Weingartner, D. F. Sauer, G. V. Dhoke, M. D. Davari, A. J. Ruff, and U. Schwaneberg, Appl Microbiol Biotechnol. 2018; 102(22): 9657–9667. doi: 10.1007/s00253-018-9328-3), we report a first screening system designed for screening of P450 variants capable to produce hydroquinones. The hydroquinone quantification assay is based on the interaction of 4-Nitrophenylacetonitrile (NpCN) with hydroquinones under alkaline conditions. The NpCN assay can be used for the quantification of dihydroxylated aromatic compounds such as hydroquinones, catechols and benzoquinones. We chose the hydroxylation of pseudocumene by P450 BM3 as a target reaction and screened for improved trimethylhydroquinone (TMHQ) formation. Here the dataset to the above mentioned manuscript is reported. Source Data of the NMR spectra (Fig1 and FigS1) are given as well as source data in Excel format to display the spectra of the hydroquinones (FigS3), the linear detection range of the assay of different hydroquinones (FigS6) or of TMHQ (Fig2) and screening data of the SSM library on position 330 with the NpCN assay (FigS7). In the description of dataset a summary of the collected source data is given.