File S1 - Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy

Detailed description of methodologies and measurements performed: Cell culture. Flow cytometry and immunofluorescence procedures. AFM indentation measurements. On the role played by cell elasticity on SCFS measurements. SCFS measurements. Table S1 in File S1: Surface immunophenotype of GASC and Gasc. Results are expressed as percentage of cells expressing the assessed marker. Student t-test: significance p<0.05. Figure S1 in File S1: SCFS measurements of GASC/Gasc subpopulation on fibronectin. Comparison of the detachment forces obtained for GASC on fibronectin and Gasc on fibronectin for increasing contact time (For a better visualization and comparison of the data, Y scale is reported as Log scale). No significant differences are observed for all the contact times investigated.
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