TWIST1 ChIP-seq analysis of human mesenchymal stem cells

Mesenchymal stem/stromal cells (MSCs) function as skeletal progenitors in bone marrow and regulate hematopoiesis and hemodynamic processes via secretion of paracrine acting factors. Although TWIST1 plays important roles in mesoderm formation and skull and vascular development, its role in MSCs is poorly defined. Therefore, we conducted ChIP-seq analysis to identify genes potentially regulated by TWIST1. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) of TWIST1 binding sites in human mesenchymal stem cells.