Comparison of different T cell differentiation states by RNA-seq analysis identifies BX-795 induced Th-IL-2 cells

We here report RNA-seq data on CD4+ T cells from double transgenic allergy-mice (Neunkirchner A. et al.) expressing a humanized T cell receptor (TCR) specific for the major mugwort pollen allergen Art v 1 peptide 23-36 in combination with a humanized HLA-DR molecule at time-point zero (FACS sorted naive CD4+ T cells from murine spleen) and after 120 h of stimulation of such FACS sorted naive T cells with bone-marrow derived dendritic cells (BMDCs) from these mice plus recombinant Art v 1 (rArt v 1) protein and either DMSO (T effector), BX-795 (Th-IL-2) or a mix of TGF-b, anti-IL-4 and anti-IFN-g (iTreg). T cells cultured for 120 h were FACS sorted before RNA extraction to exclude BMDCs (purity > 90 %). These data is supposed to characterize the expression phenotype of T cells differentiated in the presence of BX-795 (Th-IL-2) and extract specific features by comparing it to control phenotypes (naive, T effector, iTreg). RNA-seq of murine naive CD4+ T cells at time point 0 and after 120 h of stimulation at various differentiation conditions