Manual nuclei segmentations in near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of nocodazole treated HeLa cell (jrc_hela-nz-1)

Annotation description: Dense segmentations of nuclei in jrc_hela-nz-1 using Amira-Avizo (Thermo Fisher Scientific) and "Using Amira to manually segment organelles in vEM for machine learning V.3" protocol.Annotation protocol: Using Amira to manually segment organelles in vEM for machine learning V.3 (http://dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3)Software: Amira 3D 2021.1, Classic Segmentation WorkroomClasses annotated: NucleiSource (EM) dataset ID: jrc_hela-nz-1Source (EM) voxel size (nm): 8 x 8 x 7.9 (X, Y, Z)Source (EM) data dimensions (µm): 99.2 x 4.0 x 73.1 (X, Y, Z)Source (EM) DOI: https://doi.org/10.25378/janelia.24944784Dataset URL: s3://janelia-cosem-datasets/jrc_hela-nz-1/jrc_hela-nz-1.zarr/recon-2/labels/inference/segmentations/nuc/Visualization website: https://openorganelle.janelia.org/datasets/jrc_hela-nz-1