Transcriptomic analysis of mutant yeast strains deficient in Xrn1 nuclear import. Transcriptomic analysis of mutant yeast strains deficient in Xrn1 nuclear import

The goal of the project was to study the transcription rates and mRNA levels, genome-wide, in several mutants in Xrn1 defetive in nuclear import. We used Genomic Run-On (GRO) experiment in wild type and xrn1 mutant strains. Overall design: Cells were grown in YPD at 30ºC to 0.4-0.5 OD600. Cells were spun-down and collected. Note that each sample record represents three replicates .