mRNA 3'end sequencing of four human cell lines

We developed a computational tool for APA identification and quantification (APAIQ) from RNAseq data, which is capable of identifying PAS and quantifying their expression level in a genome-wide manner. To benchmark APAIQ and other published methods, we performed mRNA 3'-end sequencing on four human cell lines, including HepG2, K562, SNU398 and THLE2. In additional, a matched conventional mRNA sequencing was also performed on K562.