Gene expression signatures for rat liver grafts

Acute rejection remains an important risk factor affecting the survival of recipients following transplantation. Bone marrow mesenchymal stem cells (BMMSCs) are used in the treatment of organ transplantation due to their immunomodulatory ability. BMMSCs were isolated from rat bone marrow and modified with the adenovirus for heme oxygenase 1 (HO-1) gene. Saline solution, BMMSCs or HO-1/BMMSCs were perfused into the donor liver in vitro using a normothermic machine perfusion (NMP) system, followed by liver transplantation. The liver grafts were collected at 7 days post-transplantation. Gene chip technology was used to detect differential gene expression.急性排斥反应（Acute rejection）仍是影响移植术后受体存活的重要危险因素。骨髓间充质干细胞（Bone marrow mesenchymal stem cells, BMMSCs）因具备免疫调节能力，被应用于器官移植治疗领域。研究人员从大鼠骨髓中分离得到BMMSCs，并利用腺病毒介导血红素氧合酶1（Heme oxygenase 1, HO-1）基因对其进行修饰。随后，借助常温机械灌注（Normothermic machine perfusion, NMP）系统，将生理盐水、BMMSCs或HO-1/BMMSCs体外灌注至供体肝脏，继而完成肝移植手术。于移植术后7天收集移植肝组织，采用基因芯片技术检测差异基因表达水平。