Genome-wide bisulfite sequencing reveals enhancer as main target of DNA methyltion in neural conversion [Bisulfite-seq]. Homo sapiens

Genome-wide bisulfite sequencing and corresponding transcriptome are analyzed together to seek for methylation target during neural differentiation Overall design: H1 human embryonic stem cells are able to differentiated into neural stem cells with induction of compound C in 72h. We extracted genomic DNA from 0, 24, 48h samples during induction and measured their DNA methylation level by BS-seq.