Retinal polyunsaturated fatty acid supplementation reverses aging-related vision decline in mice

The retina is uniquely enriched in polyunsaturated fatty acids (PUFAs), primarily localized in cell membranes, where they govern membrane biophysical properties. During aging, alterations in lipid metabolism lead to reduced content of very long-chain PUFAs (VLC-PUFAs) in the retina, which is associated with normal age-related reductions in contrast sensitivity, diminished photoreceptor function and delayed rod-mediated dark adaptation recovery, and pathological age-related macular degeneration (AMD). ELOVL2 (Elongation of very-long-chain fatty acids-like 2) encodes a transmembrane protein that produces precursors to docosahexaenoic acid (DHA) and VLC-PUFAs. The methylation status of the ELOVL2  promoter is currently one of the best predictors of chronological age. Here, we show that lower VLC-PUFA abundance in the aged mouse retina is accompanied by a reduction in visual function. Similarly, mice lacking ELOVL2-specific enzymatic activity (Elovl2C234W) demonstrate reduced contrast ..., , # Retinal polyunsaturated fatty acid supplementation reverses aging-related vision decline in mice

Dataset DOI: [10.5061/dryad.gxd25480h](10.5061/dryad.gxd25480h)

## Description of the data and file structure

To investigate molecular changes retinas in aging and after lipid supplementation, total fatty acid, global lipidomic, transcriptomic, and targeted proteomic analyses were performed on mouse retinas and photoreceptor outer segments. The data in this file was used to produce panels in Figure 1, 3, 5 and 6 and Supplementary Figure 1, 3, 5 and 6 of the associated publication. In the associated publication, we utilized p-values from the multiple t test or 2-way ANOVA. This file can be opened with any text editing software. In the present study, this file was included in analyses that utilized the Prism software.

### Files and variables

#### File: retina_tFA-different_age_stages.csv

**Description:** Total fatty acid analysis was performed on mouse retinas collected from differen...,