Expression analysis of fully-grown oocyte collected from mouse wild-type, Hsf1 mutant or Hsf2 mutant

Investigation of whole genome gene expression level changes in oocytes Hsf1-/- or Hsf2-/- compared to wild-type. The mutants analyzed in this study are further described in McMillan DR, Xiao X, Shao L, Graves K, Benjamin IJ. 1998. Targeted disruption of heat shock transcription factor 1 abolishes thermotolerance and protection against heat-inducible apoptosis. J Biol Chem. 273(13):7523-8 and in McMillan DR, Christians E, Forster M, Xiao X, Connell P, Plumier JC, Zuo X, Richardson J, Morgan S, Benjamin IJ. 2002. Heat shock transcription factor 2 is not essential for embryonic development, fertility, or adult cognitive and psychomotor function in mice. Mol Cell Biol. 22(22):8005-14. A microarray study using two technical replicates of total RNA recovered from wild-type fully-grown oocytes, Hsf1 mutant fully-grown oocytes and Hsf2 mutant fully-grown oocytes. Each microarray measures the expression level of 42,586 probe sets from Mus musculus with nine 60-mer probe pairs per gene.本研究聚焦于相较于野生型（wild-type）的Hsf1基因敲除（Hsf1-/-）或Hsf2基因敲除（Hsf2-/-）卵母细胞（oocytes）的全基因组基因表达水平变化。本研究中分析的突变体的详细背景已在以下两篇文献中详述：McMillan DR、Xiao X、Shao L、Graves K、Benjamin IJ于1998年发表的《热休克转录因子1（heat shock transcription factor 1）的靶向敲除可消除耐热性及对热诱导凋亡的保护作用》，刊载于《Journal of Biological Chemistry》（J Biol Chem），273(13):7523-8；以及McMillan DR、Christians E、Forster M、Xiao X、Connell P、Plumier JC、Zuo X、Richardson J、Morgan S、Benjamin IJ于2002年发表的《热休克转录因子2（heat shock transcription factor 2）对小鼠胚胎发育、生育能力或成年认知与精神运动功能并非必需》，刊载于《Molecular and Cellular Biology》（Mol Cell Biol），22(22):8005-14。本研究采用基因芯片（microarray）技术，对野生型完全成熟卵母细胞、Hsf1突变型完全成熟卵母细胞及Hsf2突变型完全成熟卵母细胞提取的总RNA（total RNA）进行两次技术重复（technical replicates）检测。每张芯片可检测小家鼠（Mus musculus）的42586个探针组（probe sets）的表达水平，每个基因对应9条60聚体探针对（60-mer probe pairs）。