Additional file 2: Figure S1. of P2X7 receptor mediates NLRP3-dependent IL-1β secretion and parasite proliferation in Toxoplasma gondii-infected human small intestinal epithelial cells

FHs 74 Int cells infected with T. gondii at higher infectious doses (MOI 20) reduced cell viability. FHs 74 Int cells were infected with the T. gondii GFP-RH strain at the indicated MOIs for 8 h, and the viability of FHs 74 Int cells were evaluated by an MTT assay. Figure S2. Toxoplasma gondii-induced IL-1β production in FHs 74 Int cells. FHs 74 Int cells were infected with T. gondii at an MOI of 10 for different time periods, and pro-IL-1β protein levels in the cell extracts were determined by Western blotting. a Representative blots presenting the protein levels of pro-IL-1β in mock-infected or T. gondii-infected FHs 74 Int cells (α-Tubulin served as an internal control for protein loading). b Bar plot depicting the pro-IL-1β/α-Tubulin ratio as determined by densitometric analysis of Western blotting and expressed as fold change compared with the mock-infection control. For all panels, data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the uninfected control. All data are representative of three independent experiments. (ZIP 1710 kb)