Fast scanning SAXS of living biological cells

We have recently employed hard X-ray scanning SAXS in a special fast scanning mode at ID13 to image hundreds of freeze-dried biological cells in a single scan, and have then transferred the method to fixed-hydrated cell, which was an important step towards more physiological conditions. In the proposed experiment, we suggest to now apply the method to living cells. We will fabricate special flow chambers and investigate keratin-rich cells expressing particularly pronounced protein bundles, as a bench mark for studying such ordered structures.