MG-treated vs non-treated ST2 cells Mouse 32K. Mus musculus

In diabetics, methylglyoxal (MG), a glucose-derived metabolite, plays a noxious role by inducing oxidative stress, which causes and exacerbates a series of complications. With the use of microarray analysis, we comprehensively screened the gene expression profiles of ST2 cells, derived from a multipotent bone marrow stromal cell line, in the presence or absence of oxidative stress induced by100 μM methylglyoxal (MG) treatment to charactrize genes related to diabetic complications. Overall design: Mouse bone marrow stromal cell-line ST2 (RIKEN, Tsukuba, Japan) was cultured in α-MEM (Sigma, St. Louis, MO) supplemented with 10% FBS (Sigma), 100 μg/ ml penicillin/ streptomycin (ICN Biomedicals, Inc., Aurora, OH) and 100 μM methylglyoxal (MG), and maintained at 37°C in a humidified atmosphere with 5% CO2. Total RNA was isolated from ST2 cells treated with or without 100 μM methylglyoxal (MG) or 1 μM of 5-aza-2’-deoxycytidine (5-aza-dC) by standard methods with the use of an RNeasy Protect Mini kit (Qiagen KK, Tokyo, Japan) according to the manufacturer’s instructions. Cy3, Cy5 differential expression asasy by Mouse 32K Filgen array