PD1-mediated microglia assisted cerebrovascular pruning during vascular development.
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https://www.ncbi.nlm.nih.gov/sra/SRP530538
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To further explore the mechanism of cerebrovascular pruning by PD1 in microglia, we analyzed RNA isolated from Pd1fl/fl and Pd1cKO-Cx3 cerebral cortical microglia at P11 using RNA-seq to identify genome-wide changes. Overall design: mRNA from P11 isolated microglia of Pd1fl/fl and Pd1cKO-Cx3 mice was extracted. Specifically, Agilent 2100 Bioanalyze was used to quality controlled and quantified. then, mRNA was converted to cDNA and bound the library. RNA-sequencing analysis was used by the Illumina HiSeq 2500 platform in Annoroad Genomics
创建时间：
2024-09-10
